Profile of Dr. Md. Abu Sayed

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Dr. Md. Abu Sayed

Associate Professor

Department of Biochemistry & Molecular Biology (BMB)

Faculty of Agriculture

Hajee Mohammad Danesh Science & Technology University, Dinajpur.

E-mail: sayed_bmb@yahoo.com


CAREER OBJECTIVE

    To establish a modern molecular biology laboratory for doing basic and applied research by making collaboration with renowned Professors / Researchers / Scientists in Bangladesh and abroad.

RESEARCH INTEREST

    Role of mitochondrial alternative oxidase and cytosolic phosphoenolpyruvate carboxylase for mitigating abiotic and biotic stress in plants. Development of C4 rice through genetic manipulation of carbonic anhydrase, Rubisco and PEPC enzyme. Formulation of bio-pesticide and herbal medicine by Leonurus siribicus.

EDUCATION

  1. PhD, 2017

    Iwate University, Japan

  2. Visiting Research Fellow, 2013

    Nottingham University, UK

  3. Master of Science (MS) in Biochemistry, 2009

    Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

  4. B.Sc.Ag. (Hons), 2007

    Bangladesh Agricultural University, Mymensingh-2202, Bangladesh


PROFESSIONAL EXPERIENCES

  1. Associate Professor
    Dept. of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University

    May 16, 2017 to Present

  2. Assistant Professor
    Dept. of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University

    May 16, 2012 to May 15, 2017

  3. Lecturer
    Dept. of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University

    May 16, 2010 to May 15, 2012


PUBLICATIONS

Journal Papers

  1. Tahjib-Ul-Arif, M., Sayed, M.A., Islam, M.M., Siddiqui, M.N.A., Begum, S. N., Hossain, M.A. 2018. Screening for salinity stress tolerance of rice landraces (Oryza sativa L.) using morpho-physiological and molecular markers at seedling stage. Acta Physiologiae Planturam.40:70  https://doi.org/10.1007/s11738-018-2645-4 (Springer, IF: 1.681)

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  2. Siddiqui, M.N., Mostofa, M.G., Akter, M.M., Srivastava, A.K. Sayed, M.A., Hasan, M.S., Tran, L.S.P. 2017. Salt-induced toxicity impacts on growth and yield-potential of local wheat cultivars: Oxidative stress and ion toxicity are among the major determinants of salt-tolerant capacity. Chemosphere.187:385-394 (Elsevier, IF 4.506)

  3. Ali, M.T., Alam, M. A., Ullah, M.E., Ashraf, M. A., Sayed, M.A., Jahan, A. 2017. Prediction of possible effects of arsenic and cadmium in human health using chemical-protein and protein-protein interaction network. Journal of Chemical, Environmental and Biological Engineering. 2(4):45-52.

  4. Ali, M.T., Sazed, S.A., Palit, P., Sayed, M.A, Aalm, M.A. 2017. Network and pathway analysis for Cesium in Homo sapiens. Bioresearch Communications. 3(1): 289-297.

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  5. Sayed, M.A., Yui, U, Ito, K. 2016. Metabolic interplay between cytosolic Phosphoenolpyruvate carboxylase and mitochondrial alternative oxidase in the thermogenic skunk cabbage, symplocarpus renifolius. Plant Signaling & Behavior. 11(11): e1247138 (Tylor and Francis)

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  6. Sayed, M.A., Alam, M. A.,  Islam, M.S., Ali, M.T., Ullah, M.E., Shibly, A.Z., Md. Aslam Ali, M.A., Olive, M.M.H. 2016. Leonurus siribicus L. (Honeyweed): A review of Its Phytochemistry and Pharmacology. Asian Pacific Journal of Tropical Biomedicine. 6(12):1076-1080 (Elsevier).

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  7. Roy, P.R., Tahjib-Ul-Arif, M., Akter, M.T., Ray, S.R., Sayed, M.A. 2016. Exogenous ascorbic acid and hydrogen peroxide alleviates salt-induced oxidative stress in rice (Oryza sativa L.) by enhancing antioxidant enzyme activities and proline content. Advances in Environmental Biology. 10(10):148-154 (ISI, Thomson Reuters)

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  8. Sayed, M. A., Imam, R., Haque, M. M., Siddiqui, M. N. A., Raihanun-Nabi, S.M.,   Aktar, S., Das, S. R., Hossain, M. A. 2015. Allelopathic activity Leonurus siribicus L. on seed germination and seedling growth of wheat in vitro and 4- hydroxy benzoic acid is an allelochemical determination by chromatography. Pakistan Journal of Botany. 48 (3): 1189-1195 .(SCIE, IF: 0.822)

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  9. Islam, M.S. Siddiqui, M.N., Sayed, M.A., Tahjib-UL-Arif, M., Islam, M. A., Hossain, M.A. 2016. Dietary effects of buckwheat (Fagopyrum esculentum) and black cumin (Nigella sativa) seed on growth performance, serum lipid profile and intestinal microflora of broiler chicks. South African Journal of Animal Science. 46(1):103-111. (SCIE, IF: 0.504)

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  10. Sayed, M. A., Islam, M. T., Haque, M. M., Hossain, S. M. J., Uddin, R., Siddiqui, M. N. and Hossain, M. A. 2015. Dietary effects of chitosan and buckwheat (Fagopyrum esculentum) on the performance and serum lipid profile of broiler chicks. South African Journal of Animal Science. 45(4): 429-440. (SCIE, IF: 0.504)

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  11. Siddiqui, M. N., Islam, M. T., Sayed, M. A. and Hossain, M. A. 2015. Effect of Dietary Supplementation of Acetone Extracts of Nigella sativa seeds on Serum Cholesterol and Pathogenic Intestinal Bacterial Count in Broilers. The Journal of Animal and Plant Sciences. 25 (2): 372-379. (SCIE, IF: 0.422)

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  12. Islam, M. T., Selim, A. S. M., Sayed, M. A., Khatun, A., Siddiqui, M. N. A., Alam, M. S. and Hossain M. A. 2011." Nigella sativa L. supplemented diet decreases egg cholesterol content and suppresses harmful intestinal bacteria in laying hens. Journal of Animal and Feed Sciences. 20 (4). 587-598. (SCIE, IF: 0.75)

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  13. Siddiqui, M.N. and Sayed, M.A. 2015. Effect of dietary black seed (Nigella sativa L.) extract supplemented diet on growth performance, serum metabolites and carcass traits of commercial broiler. Journal of Animal Science Advances. 5(8):1380-1385.

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  14. Rashid, M., Islam, M.S., Salahuddin, M., Sayfullah, M., Hussain, D., Momin, M.A., Sayed, M. A., Sah, J.P., Sah, S.K. 2015. The Biochemistry of hunger stimulating hormone: Why understanding this cascade in hypothalamus is beneficial. Biochemistry and Physiology. 4(3): e136.

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  15. Sah, J.P., Salahuddin, M., Sayed, M.A., Salauddin, S.A., Syfullah, M., Rashid, M., Saclain, S., Washim, M.R., Zeb, M.A., Hossain, D., Chowdhury, M.R., Momin, M.A. 2015. Transfection optimization in HEK-293 cell line. Asian Journal of Medical and Biological Research. 1(1): 121-129.

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  16. Mondal, M.A., Yeasmin, T.,Karim, R., Siddiqui, M.N., Raihanun-Nabi, S. M., Sayed, M.A. 2015. Effect of dietary supplementation of turmeric (curcuma longa) powder on the growth performance and carcass traits of broiler chicks. SAARC Journal of Agriculture. 13(1): 188-199.

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  17. Hossain, A. K. M. S., Latif, M. A., Biswas, B. K., Saclain, S., Salahuddin, M., Sayed, M. A., Hussain, M. M., Moniruzzaman, M. S. and Islam, M. S. 2014. Extraction of Jute Genomic DNA: Difficulties and Solutions. International Journal of Applied Sciences and Biotechnology (IJASBT). 2(4): 516-520

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  18.  Hossain, A., Sayed, M. A., Sarker, B. C., Mojumder, U. K. and Hossain, M. A. 2013. Prevalence and Risk factors with diabetes mellitus among the people in Dinajpur district of Bangladesh. J. Sci. Technol. 11:80-85.

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  19. Islam, R., Anwar, M.B., Hakim, M.A., Khan, M.M., Sayed, M.A. 2013. Screening of heat tolerant wheat Genotypes in Bangladesh. Journal of Natural Products.6: 132-140.

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  20. Sayed, M. A., Islam, M. T., Haque, M. M., Hossain Shah, M.J. and Hossain, M. A. 2013. Buckwheat supplemented diet suppresses serum triglycerides and increases high density lipoprotein in broilers for antibiotic free safe meat. Science Secure Journal of Biotechnology. 2 (1):26-35.

  21. Ahad, M. A., Sayed, M. A., Siddiqui, M. N. A and Haque, M. M. 2012. Evaluation of some indigenous plant extracts against pulse beetle, Callosobruchus chinensis L. (Bruchidae: Coleoptera) in stored green gram (Vigna radiata L.). Global Journal of Medicinal Plant Research. 1(1):33-41.

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  22. Ali, M. A., Hasan, S. M. K., Mahmud, M. S. and Sayed, M. A. 2012. Processing and storage of instant cooked rice. Bangladesh Res. Pub. J. 7(3):300-305.

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  23. Sayed, M. A., Haque, M. M., Roy, B., Hossain, S. M. J. and Das, S. R. 2012. Allelopathic Effects of different extracts of honeyweed (Leonurus siribicus L. on seeds germination and seedlings growth of some selected vegetables. Journal of Natural Products.5: 243-250.

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  24. Aktar, S.,  Sayed M. A., Islam, M. R., Roy, B. and Hossain, M. A. 2012. Growth regulatory activities of different extract of Tinospora cordifolia on some vegetable seeds with their chemical investigation. J. Environ. Sci. & Natural Resources. 5(1): 129-132.

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  25. Roy, B., Sarker, B. C., Ali, M. R., Das, S. R., and Sayed, M. A. 2012. Seed germination and seedling growth of two vegetables in responses to aqueous extract of four herbal plant leaves. J. Environ. Sci. & Natural Resources. 5(1): 133-140.

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  26. Farid, M. S., Mamun, M. A. A., Matin, M. A., Jahiruddin, M. and Sayed, M. A. 2011. Effect of cowdung, poultry manure, dhaincha and fertilizers on the nutrient content and uptake of BRRI dhan 41. Bangladesh J. Prog. Sci. & Tech. IX (2): 209-212.

  27. Paul, T., Mozumder Rubel, N. H. M., Sayed, M. A., and Akhtaruzzaman, M. 2011. Proximate compositions, mineral contents and determination of protease activity from green gram (Vigna radiata L. Wilczek). Bangladesh Res. Pub. J. 5(3):207-213.

  28. Sayed, M. A., Mortuza, M. G., Rashid, M. H. and Hossain, M. A. 2009. Formulation of stevia incorporated cereal-legume based therapeutic food for regulating lipid profile. J. Agrofor. Environ. 3(2):95-97.

  29. Sayed, M. A., Mortuza, M. G., Rashid, M. H. and Hossain, M. A. 2009. Growth Performances of Rat Fed on Formulated Food Added with Stevia. J. Environ. Sci. & Natural Resources. 2(2):189-192.

Conference Papers

  1. Sayed, M. A., Umekawa, Y. and Ito, K. Isolation and expression analysis of a cDNA encoding for phosphoenolpyruvate carboxylase in thermogenic skunk cabbage, Symplocarpus renifolius. Proceedings in the International Conference on Biochemistry held from 10-12 October, Kuala lumpur, Malaysia.

  2. Sayed, M. A., Umekawa, Y. and Ito, K. Metabolism of phosphoenolpyruvate in thermogenic spadix of skunk cabbage, Symplocarpus renifolius. Proceedings in the 38th Annual Meeting of Biochemistry and Molecular Biology (BMB2015), Kobe, Japan held from 1-4 December, 2015.

  3. Umekawa, Y., Sayed, M. A. Seito, T., Ito, K. Analysis of temperature effects on NADH respiration of isolated mitochondria from thermogenic tissue in skunk cabbage. Proceedings in the 87th Annual Meeting of the Japanese Biochemical Society: Biochemistry Underlying Biomedical Innovation, Kyoto, Japan held from 15-18 October, 2014.

  4. Umekawa, Y., Seito, T., Sayed, M. A., Ito, K. The rotenone-insensitive internal alternative NADPH dehydrogenase- dependent respiration in the mitochondria from skunk cabbage: temperature responses of COX-and AOX-mediated respiration pathways. Proceedings in 18th European Bioenergetics Conferences (EBEC), Lisbon, Portugal held from 12-17 July, 2014.

  5. Ghori, V., Fox, A. J., Noori, H., Santidamrongkul P., Sayed, M. A. and Leach, L. L-NAME alters sub-cellular distribution of eNOS and caveolin-1 in the perfused human placental microvascular bed. Proceedings on Conference of International Union of Physiological Sciences (IUPS), Birmingham, United Kingdom held from 21-26 July, 2013.

  6. Sayed, M. A., Islam, M. T., Haque, M. M., Hossain, M. J., and Hossain, M. A. Buckwheat supplemented diet suppresses serum triglycerides and increases high density lipoprotein in broilers for antibiotic free safe meat. Proceedings in International Conference on Transfer of Biotechnology and Land Use Management for Sustainable Development, Government College of Aron, Madhya Prodesh, India held from 16-17 February, 2013.

  7. Siddiqui, M. N. A., Islam, M. T., Sayed, M. A., and Hossain M. A. Supplementation of acetone extract of Nigella sativa L. seeds in diet decreases serum cholesterol and harmful intestinal bacteria in broiler. Proceedings on Conference of the Bangladesh Society for Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh held from 25-26 February, 2012.

  8. Roy, B., Sarker, B., Ali, M. R., Das, Shukla. R., and. Sayed, M. A. Seed Germination and Seedling Growth of Some Vegetables in Response to Aqueous Extract of Four Plant Leaves. Proceedings on Third International Seed Conference: Quality Seed for Food Security under Changing Climate. Bangladesh Agricultural University, Bangladesh held from 8-10 February, 2012.

  9. Islam, M. T., Siddiqui, M. N. A., Sayed, M. A., Selim, A. S. M., and Hossain M. A. Nigella sativa L. Supplemented Poultry Feed for Antibiotic Free Low Cholesterol Egg and Safe Meat. Proceedings on Conference of the Chittagong Veterinary and Animal Science University, Bangladesh held from 7-8 March, 2012.

Books

  1. Sayed, M. A. and Hossain, M. A: Stevia Supplemented Diet for Management of Cardiovascular Disease. LAP Lambert Academic Publishing GmbH & Co. KG, Germany, 2011.


SCHOLARSHIPS

  1. Japanese Government (Monbukagakusho: MEXT) Scholarship for PhD study from April 2014-March 2017.

    Funded by: Ministry of Education, Culture, Sports, Science and Technology, Japan

  2. Japanese Government (Monbukagakusho: MEXT) Scholarship as a Research Student from October 2013-March 2014.

    Funded by: Ministry of Education, Culture, Sports, Science and Technology, Japan

  3. Academic Visitor under the 2013 Visiting Fellowship Scheme

    Funded by: The University of Nottingham, UK

  4. Academic Visitor under joint British Council INSPIRE Strategic Partnership Project SP-0112 in 2012

    Funded by: British Council

  5. NSICT fellowship (Now NST) in 2008.

    Funded by: Ministry of Science and Technology, Bangladesh

  6. Pabna Zilla Parishod Meirt Scholarship in 2000.

    Funded by: Pabna Zilla Parishod, Pabna, Bangladesh

  7. Primary School Merit Scholarship in 1993.

    Funded by: Primary Education Board, Bangladesh


PROJECTS

  1. Value addition and standardization of nutritional level in selected food items from animal and plant origin

    Funded by: Project Implementation Unit(PIU), NATP: Pahse II Prtoject, BARC, Farmgate, Dhaka-1215, Bangladesh

    Position: Co-Investigator

    Description: Growing population and rising household income have led to rapid increasing demand for food products, especially meat, milk and eggs in Bangladesh over the last two decades. Together with innovations on the supply side, the search for the most viable protein sources has resulted in particularly rapid growth of industrial poultry production along with small-scale enterprises. Among the major concerns related to this development are health issues threatening not only animal production, but also the people using the products derived from these animals. Intensification has brought food-safety concerns into sharper focus (Blancou et al. 2005), and these concerns have been increasingly acknowledged, at least in developed countries, as information technology and medical science have advanced (Nelson, in FAO 2005). With the increment in the per capita demand and supply for meat and fish products in developing countries, the new intensive production systems of the developing world are facing more and more pressure to comply with the regulations that prevail in the global market. Food safety nowadays has become an important topic in Bangladesh as consumers of the country have become victim due to serious adulteration in food. It can be referred as the system that keeps food and food products free from substances hazardous to human health that again refers to any biological, chemical or physical property that may cause unacceptable risk (FAO 1998). The emergence and discovery of new food-borne pathogens and other food-related hazards has increased the need for food-safety measures. Food security is a complex issue, where livestock and poultry products are generally regarded as high risk commodities with respect to microbial, chemical and other possible contaminants and adulterants (Yousuf et al. 2008 and Ukut I-OE et al. 2010). Food safety should be a part of governments’ strategies to ensure secure food for the consumers. Poultry meat and eggs are the two popular and easy-to-afford protein sources to the people of Bangladesh. This cheaper poultry specially chicken meat is an important part of the daily diet and accounts for 75 percent of the national demand for meat in Bangladesh (IRIN 2014). But recent reports revealed that these food items are not safe anymore as chickens are regularly and extensively exposed to various toxic chemicals (specially from tannery wastes), antimicrobial and some other drugs (The Daily Star 2014, Hossain and Hasan 2014) and some growth promoters/hormone derivatives. They found chromium, a heavy metal levels ranging from 249 to 4561g per kg in chickens that consumed feed manufactured with tannery waste against the permissible limit is 10-60g. The excess amount of chromium can be transported from poultry feed to the human body through the chicken leading to the carcinogenic effects on human beings like cancer, ulcer, liver cirrhosis and kidney damages, etc. About 30.7% of fish meal, 52.4% of meat and bone meal and 56.3% of meat meal samples in Bangladesh are adulterated with leather meal (BARC 2014). Some findings of BARC in 2012 and 2013 indicated that about 48 percent poultry feed contains tannery waste and presence of antibiotic residues in eggs and chicken were far beyond the acceptable limit (cited in The Daily Star 2014). Therefore, antibiotic resistance is developing day by day among the people of Bangladesh. Continuous ingestion of higher level of antimicrobial drugs through animal food origin is contributing to the antimicrobial resistance problem by creating a resirvour of resistance bacteria (Bailar and Travers 2002 and O’Connor et al. 2002) constituting health risks to the consumers. In fact, most of the chemicals and veterinary drug residues, either from feed or other exogenous uses, in animal foods may have some noxious effects on human health. Croubels et al. (2004) reported that some of these residues may exert genotoxic, immunotoxic, carcinogenic or endocrine effects in human body. Like fruit and vegetables, the use of health-hazard materials in livestock and poultry products became a serious issue in Bangladesh in the recent years. But, their usages are not been controlled. This could be due to weak business policy, lack of awareness of producers, excessive dependency on non-technical traders, carelessness of some technical personnel, inadequate policy guidelines and lack of strong monitoring of law enforcing agencies, etc. Use of tannery waste makes poultry feed cheap while antibiotics help reduce deaths of chicks. These practices pose serious health risks to consumers already worried at reports of formalin and other chemicals in food. The whole nation is under threat as chicken meat and egg are the most consumed and also the cheapest source of animal protein (Hossain and Hasan 2014). Human specially children consuming poultry meat and egg are claimed to become fattened and gained unusual growth that could be due to residual effects of antibiotic and/or growth promoters. HACCP is a system of extensive evaluation and control over an entire food production process for the sole purpose of reducing potential food-related health risks to consumers. It is a world-wide recognized systematic and preventive approach that addresses biological, chemical and physical hazards through anticipation and prevention, rather than through end-product inspection and testing and thereby reducing the food-borne illness (Gandhi 2009). The hazards associated with meat and poultry products may differ in various regions of the world (Tompkin 1994) because of the differences in environment and practices in different areas. Hoque et al. (1997) found a 10% prevalence of Salmonella in commercial poultry farms in Bangladesh. Although the country has animal food safety regulation those are rather theoretical than practice. Under these circumstances, poultry feeds and poultry products need to be analysed. Efforts should be given to identify the extent and steps of use of the hazardous materials, chemicals and their derivatives, and microbial contamination in poultry feed and their residual effects in raw and value added poultry products. Critical evaluation should be done on HACCP practices on poultry origin food chains. Moreover, nutritive composition of the commercial poultry diets are also worthwhile to be investigated whether the farmers are cheated or not by the feed manufacturers. Earlier reports indicate that nutritional composition of poultry feed significantly affect growth performances of broilers (Kamran et al. 2008, Dairo et al. 2010) and layers (Gunawardana et al. 2008, Li et al. 2013 and Nahashon et al. 2007). The present study will help to formulate a guideline to the policy makers and law-enforcing agencies to minimize or stop these unethical practices concerning safe food production as well as building healthy nation.

  2. Effect of honeyweed (Leonurus siribicus) supplemented diet on serum cholesterol and harmful intestinal bacteria in broiler

    Funded by: Institute of Research and Training, HSTU

    Position: Principle Investigator

    Description: Antibiotics as feed additives have been used for years to improve the profitability of poultry production by helping to control pathogenic bacteria in the gut mucosa, thereby improving weight gain, feed conversion ratio, and uniformity. However, the development of direct antibiotic resistance of pathogens in the species receiving the feed, as well as the indirect resistance to similar antibiotics used in human medicine as the result of food chain residues, led to the ban of all sub-therapeutic levels of growth promoting antibiotics by EU and many other countries including Bangladesh. Removal of antibiotics from the diet may negatively affect profitability of the poultry industry. Therefore, there is a great interest in developing natural alternatives to antibiotic growth promoters in order to maintain both bird performance and health. One of the alternatives to synthetic antibiotic feed additives in poultry feed could be the herbal medicinal plant, Honeyweed (Leonurus siribicus). Leonurus sibiricus (L. sibiricus) is a ubiquitous herbaceous plant grown in crop fields in many countries in Asia and South America. This plant belongs to the family of Lamiaceae. It is an annual, biannual or perennial, aromatic, herbaceous plant. The average height is 40–120 cm and it germinates from seed. Lamiaceae family consists of about 236 genera and 6900–7200 species. Among all the genera, Leonurus contains about 20 species and L. sibiricus is one of them, which is called honeyweed or Siberian motherwort in English. It is called Rokdrone in Bengali, Guma in Hindi, Kacangma in Malay, Erva-de-Maca´e in Brazil, Marihuanilla in Mexico and marijuana in Spanish. This plant has been used as herbal medicine and culinary ingredients. L. sibiricus acts as an effective therapeutic against diabetes, menstrual irregularities, and bronchitis. Studies show that it has medicinal effects on endometritis, myocardial cells and diabetes. Although honeyweed has been used as an herbal drug for human for many years, there are no published data available on its use for poultry feed. So, honyweed may be alternative to antibiotic and low input environment safe feed additives. The proposed research will be carried out to full fill the following objectives: a.The effect of formulated diet on lipid profile (Total cholesterol, Triglyceride and HDL) of broiler. b.The effect of formulated diet on growth performances and harmful intestinal bacteria of broiler.

  3. Assesement the role of mitochondrial alternative oxidase(AOX) gene of wheat cultivars for salinity tolernace in Bangladesh

    Funded by: Institute of Research and Training, HSTU

    Position: Principle Investigator

    Description: Bangladesh is one of the members of developing countries of the third world with 160 million population. Recently, Government of the People’s Republic Bangladesh has undertaken the promotion of academic innovation in universities and research institutions to develop new crop varieties and technologies to meet the food demand for ever-increasing population in Bangladesh within the limited agricultural land area. Bangladesh is a sub-tropical country where wheat is second growing crop and grown in a short winter ranges from November to March in south western and northern region in Bangladesh. With its high population density, there is no scope of new agricultural lands being used for the increased production of wheat that is needed. One alternative is to use unfavorable land, which remains fallow, for most of the year such as the salt-affected coastal areas in Bangladesh. The severity of salinity problem in Bangladesh increases due to climate change and the desiccation of the soil. Therefore, there is an urgent need to raise salt-tolerant varieties that can maintain optimum yield levels in meeting the ever-increasing food and feed requirements. The assortment of salt-tolerant wheat varieties may prove the best approach to bring these areas under cultivation. The proposed research will be carried out to full fill the following objectives: a.The effect of different concentration of salt on agronomical, physiological and biochemical parameters of wheat cultivars. b.The effect of different concentration of salt on mitochondrial alternative oxidase (AOX) gene in wheat cultivars. c.Selection of the best variety or varieties for further molecular breeding for field production in the salinity prone areas in Bangladesh


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