Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur, Bangladesh

Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur, Bangladesh

Government Azizul Haque College, Bogura, Bangladesh

Chalitadanga B.B.N High School, Sirajganj, Bangladesh

January 15, 2026 to Present

January 07, 2025 to May 30, 2025

April 02, 2023 to April 30, 2024

Haque, Md. Samiul, Mobarak, Md. Hosne, Basher, Md. Khayrul, Sarkar, Sumon, Sarker, Sourav, Islam, Md. Rashedul, Ameliorative Potential of Vitamin E and Selenium Against Di-2-Ethylhexyl Phthalate (DEHP)–Induced Toxicity in Adult Female Mice, Veterinary Medicine International, 2025, 6844730, 12 pages, 2025.

Basher MK, Sarkar S, Sarker S, Islam MR (2025). Ameliorative potential of selenium against arsenic-induced interrupted reproductive cycle and pregnancy loss of Swiss albino female mice. J. Anim. Health Prod. 13(4): 1168-1175.

Basher MK, Sarkar S, Haque MS, Sarker S, Islam MR (2024). Protective effect of selenium against arsenic-induced hematological, biochemical alteration, and organ development anomalies in adult female mice. Adv. Anim. vet. Sci., 12(6): 1107-1116.

Md. Khayrul Basher, Sumon Sarkar, Md. Hosne Mobarak, Md. Rashedul Islam. 2023. Inorganic arsenic exposure during pregnancy affects post-natal growth, blood parameters, and organ development of mice offspring. J Adv Biotechnol Exp Ther. 6(2): 445-457.

Md. Khayrul Basher, Sumon Sarkar, Md. Rashedul Islam. 2023. The ameliorative effect of selenium on inorganic arsenic-induced estrous cycle disturbance, pregnancy complications, defective fetal and post-natal growth in mice. Proceedings of the 5th International Conference on Biotechnology in Health and Agriculture held in Dhaka, Bangladesh from September 01, 2023, to September 03, 2023.

Md. Samiul Haque, Md. Khayrul Basher, Md. Rashedul Islam. 2022. Toxic effects of inorganic arsenic on body growth and different organs of adult female mice. Journal of Bangladesh Academy of Sciences (2022); 46 (Suppl 1) pp 100.

Md. Khayrul Basher, Md. Samiul Haque, Md. Rashedul Islam. 2022. Inorganic arsenic exposure during pregnancy affects post-natal growth, blood parameters and organ development of mice offspring. Journal of Bangladesh Academy of Sciences (2022); 46 (Suppl 1) pp 49.

Md. Khayrul Basher, Sumon Sarkar, Md. Rashedul Islam. 2022. Impact of inorganic arsenic on estrous cycle, pregnancy outcomes, and post-natal growth in mice. Proceedings of 1st Jamal Nazrul Islam National Conference-2022 for Young Researchers, held in Chittagong University on 21 May 21, 2022.

Funded by: Ministry of Science and Technology, Bangladesh

Funded by: Faculty of Veterinary and Animal Science, HSTU

Funded by: Ministry of Science and Technology, Government of the People’s Republic of Bangladesh

Position: Research Assistant (RA)

Description: The proposed research project is envisioned to uncover the influence of gender on the transgenerational effects of inorganic arsenic in body growth, blood profile, and organ development in a mice model. After experimentation, it is expected to disclose the transgenerational effects of inorganic arsenic as well as the influence of gender on it, which will be considered the success of this proposed project.

Funded by: Institute of Research and Training (IRT), HSTU, Dinajpur-5200, Bangladesh

Position: Research Assistant (RA)

Description: This research project is expected to reveal the toxic effect of sodium arsenite exposure during pregnancy on postnatal growth, blood indices, & organ development of mice offspring and their potential amelioration using sodium selenite. The research findings will be helpful for policymakers to take a new approach to arsenic-induced toxicity. It is also possible to customize a permissible dose of arsenic in drinking water to create awareness among people to get a healthy generation. This information will further create new prospects for scientists to project an alternative amelioration strategy to minimize arsenic-induced toxicity.

Funded by: Ministry of Science and Technology, Government of the People’s Republic of Bangladesh

Position: Research Assistant (RA)

Description: Arsenic-induced toxicity is considered one of the major health concerns worldwide. The increasing toxicity of arsenic is alarming for the environment and poses a huge threat to global public health. To determine the mode of action of the toxicant, knowledge of the physiological action of the respective toxicant helps to anticipate crucial sub-lethal effects, and analyses of biochemistry, hematology, and histopathology may be utilized. On the other hand, sodium selenite (Na2SeO3) as a potential antioxidant plays a significant role in the amelioration of NaAsO2-induced toxicity. The present study investigated the protective potential of Na2SeO3 on NaAsO2-induced hematological, biochemical, and organ development anomalies in adult female mice. Initially, to diagnose the structural and functional status of an individual exposed to a toxicant blood parameter was analyzed and it was observed that Na2SeO3 could rescue the NaAsO2-induced hematological abnormalities. Then, to detect tissue damage and biomarkers of animals exposed to chronic concentrations of a toxicant, enzyme activities were monitored, and with fewer exceptions, all the biochemical parameters (RBS, Alkaline phosphatase, Total Cholesterol, Triglyceride) were improved after Na2SeO3 treatment. In addition, the evaluation of organs (based on morphology) showed the liver and lungs were severely affected in NaAsO2-exposed mice, which was minimized by Na2SeO3 supplementation. On the other hand, organ to body weight ratio showed that Na2SeO3 minimizes the toxic effects of Na-arsenite (NaAsO2) on different organs. Overall, these results suggest that Na2SeO3 can be potential against NaAsO2-induced hematological, biochemical, and organ development anomalies in adult female mice, and further experimentation is needed to clarify the effect of NaAsO2 in the later life of offspring as well as their amelioration strategy.

Funded by: Institute of Research and Training (IRT), HSTU, Dinajpur-5200, Bangladesh

Position: Research Assistant (RA)

Description: Arsenic-induced toxicity is considered one of the major health concerns worldwide. It is now apparent that chronic arsenic exposure disrupts fetal growth and development both in humans and animals. However, the effect of inorganic arsenic on body growth which further reflects organ development and functions is largely unexplored due to the lack of appropriate experimental model systems. Thus, the present study investigated the toxic effects of inorganic arsenic on body growth and organ development using a mice model. Mice were randomly divided into five groups and exposed to NaAsO2 at 0, 1, 10, 50, and 100 micro molars through drinking water. Initially, it was observed that a higher dose of sodium arsenite interrupts the body growth in mice. Then the body weight gain and rate of body weight gain were compared weekly basis and found that in the 5th week all the treatments showed an increasing trend in this study. Regarding the effect of NaAsO2 on organ development, evaluation of organs showed the liver and lungs were severely affected in NaAsO2 exposed mice, whereas the spleen, kidney, heart, and uterus were morphologically normal both in control and NaAsO2 exposed mice. Furthermore, organ weight and organ-to-body weight were evaluated, and NaAsO2 showed a deleterious effect on organ and organ-to-body weight. Overall, findings suggest that inorganic arsenic interrupts mice's body growth and organ development.

Funded by: Ministry of Science and Technology, Government of the People’s Republic of Bangladesh

Position: Research Assistant (RA)

Description: Sodium arsenite (NaAsO2) as an endocrine disruptor interrupts reproduction and poses a risk for offspring’s health outcomes later in life. Sodium selenite (Na2SeO3) as a potential antioxidant plays a significant role in the amelioration of NaAsO2 induced toxicity. The present study investigated the ameliorative effect of Na2SeO3 on NaAsO2 induced reproductive cyclicity, pregnancy initiation, pregnancy outcomes, and post-natal (birth to weaning) growth of offspring in mice. Initially, reproductive cyclicity was evaluated and found that Na2SeO3 could minimize estrous cyclicity interruption induced by NaAsO2. Then mice were evaluated for pregnancy initiation based on the presence of conceptuses in the uterine horn and placenta formation at gestation day 12.5. Results showed Na2SeO3 could rescue the NaAsO2 induced pregnancy loss, and the number of conceptuses was significantly (p=<0.002) higher than NaAsO2 exposed mice. Evaluation of conceptuses, placenta, and embryos showed morphologically normal features except for Na2SeO3 treated mice where a rare phenomenon of monochorionic-diamniotic twins was observed. In addition, the evaluation of organs (based on morphology) showed the liver and lungs were severely affected in NaAsO2 exposed mice, which was minimized by Na2SeO3 supplementation. On the other hand, the spleen, kidney, and heart were morphologically normal in all exposed mice. Once we observed that NaAsO2 influences placenta formation at E12.5, we initiated NaAsO2 exposure at gestation day 12.5 until parturition to examine the pregnancy outcome. Results showed that litter size and litter weight was improved after Na2SeO3 treatment, although the differences were not at a significant level. On contrary, weight/pups were reduced in Na2SeO3 treated mice when compared with NaAsO2 treated mice. Then the study evaluates the post-natal (birth to weaning) growth performance of offspring obtained from control, NaAsO2, NaAsO2+Na2SeO3, and Na2SeO3 treated mice. Results showed that the final body weight, total body weight gain, and rate of body weight gain were significantly (p=<0.01) hampered in offspring obtained from NaAsO2 treated mice than offspring of non-treated control mice, and Na2SeO3 could not minimize the effects of NaAsO2 in this study. Data revealed that in offspring obtained from both control and NaAsO2 treated groups, the rate of body weight gain was significantly (p=<0.005, p=<0.02 respectively) higher in males than females. Additionally, in offspring obtained from NaAsO2+Na2SeO3, and Na2SeO3 treated group mice, the rate of body weight gain was higher in males than females, although the differences were not at a significant level. Overall, these results suggest that Na2SeO3 can be potential against NaAsO2 induced reproductive toxicity, and further experimentation is needed to clarify the effect of NaAsO2 in the later life of offspring as well as their amelioration strategy using Na2SeO3.

Funded by: Institute of Research and Training (IRT), HSTU, Dinajpur-5200, Bangladesh

Position: Research Assistant (RA)

Description: Inorganic arsenic (NaAsO2) as an endocrine disruptor interrupts reproduction and poses a risk for offspring’s health outcomes later in life. The study investigated the effect of NaAsO2 on reproductive cyclicity, placenta formation, pregnancy outcomes, and post-natal (birth to weaning) growth of offspring in mice. Initially, reproductive cyclicity was evaluated and found that NaAsO2 interrupts the estrous cyclicity and the estrus stage was significantly (p=<0.05) influenced by NaAsO2. Then mice were evaluated for pregnancy and placenta formation at E12.5. Results showed the number of conceptuses was significantly (p=<0.001) higher in control than NaAsO2 exposed mice. Evaluation of conceptuses, placenta, and embryos showed morphologically normal features. In addition, evaluation of organs showed the liver and lungs were severely affected in NaAsO2 exposed mice, whereas the spleen, kidney, and heart were morphologically normal both in control and NaAsO2 exposed mice. Once we confirm that NaAso2 influences placenta formation at E12.5, we initiated NaAsO2 exposure at gestation day 12.5 until parturition to examine the pregnancy outcome. The litter size and litter weight were comparatively higher in NaAsO2 exposed mice, although the differences were not at a significant level. Similarly, weight/pups were significantly (p=<0.01) higher in NaAsO2 exposed mice compared to control mice. The organ morphology after parturition showed the liver and lungs were severely affected in NaAsO2 exposed mice. Finally, post-natal (birth to weaning) evaluation showed the final body weight (p=<0.002), total body weight gain (p=<0.001), and rate of body weight gain (p=<0.001) hampered significantly in offspring obtained from NaAsO2 exposed mice. Additionally, the rate of body weight gain was significantly (p=<0.005, p=<0.02 respectively) higher in males than females. Overall, findings suggest that inorganic arsenic interrupts reproduction in mice and further experimentation is needed to clarify the effect of inorganic arsenic in the later life of offspring as well as their amelioration strategy.